Effect of Deptor KD on C2C12 myoblast proliferation. Proliferation rate was determined in stably transfected myoblasts with scramble and Deptor KD. Myoblasts were seeded at the same density and counted using the Coulter Counter, as described in Materials and Methods. Time intervals are indicated; n = 6 for each treatment time point; experiments were repeated at least three times. *P < 0.05, compared with the time-matched control value. (B) Proliferation rate was measured using an independent MTT assay. Cells were treated with MTT for 4 h, and formazan produced was measured colorimetrically. Bar graph is mean ± SE; n = 16–18 for each condition, *P < 0.0001; experiments were repeated at least three times.