Deptor Knockdown Enhances mTOR Activity and Protein Synthesis in Myocytes and Ameliorates Disuse Muscle Atrophy

Table 1 Real-time PCR primers used in this study.

Gene Symbol	Gene name	Assay ID^a	PubMed reference sequence
Actb	Actin, beta	Mm01205647_gl	NM_007393.3
Gapdh	Glyceraldehydes-3-phosphate dehydrogenase	Mm999999915_gl	NM_008084.2
Rpl32	Ribosome protein L32	Mm02528467_gl	NM_172086.2
B2m	β-2 microglobulin	Mm00437762_ml	NM_009735.3
Depdc6 (Deptor)	DEP domain containing 6	Mm01195339_ml	NM_145470.2 and NM_001037937.2
Hprt	Hypoxanthine guanine phosphoribosyl transferase1	Mm00446968_ml	NM_013556.2

^aPrimers were purchased from Applied Biosystems. Assay ID represents specific details of primer sequences used.

ISSN: 1528-3658