Figure 4

Western blot and immunofluorescence analysis of the active JNK (p-JNK) and NF-κB (p-p65) expression induced by low shear stress for 12 h in HUVECs. (A) Western blot analysis of JNK protein expression (p-JNK and t-JNK) and (B) active NF-κB (p-p65) of control and low shear stress groups. (C) Quantification of activity of JNK (p-JNK/t-JNK) before and after low shear stress stimulation. (D) Quantification of p-p65 relative to ß-actin before and after low shear stress stimulation. (E) Representative immunofluorescence images by laser scanning microscopy showing p-JNK staining (green) of HUVECs before and after low shear stress for 12 h. (F) Representative immunofluorescence images showing p-p65 staining (green) of HUVECs. DAPI blue staining of cell nuclei is shown. Bar = 20 µm. (G) Quantification of relative p-JNK level by measuring the positive green area of two groups. (H) Quantification of p-p65 relative level to control. All relative expressions were normalized to the control group. *P < 0.05 versus control.