Activity of NF-κB (p-p65) and VCAM-1 expression detected by immunofluorescence and Western blot analysis with or without downregulation of JNK before and after low shear stress treatment. (A) Representative immunofluorescence images of p-p65 expression staining (green) in HUVECs with different stimulations. DAPI blue staining of cell nuclei. Bar = 20 µm. (B) Activity of p-p65 in different groups detected by immunofluorescence (IOD) was normalized to control of static group. (C) Western blot analysis of p-p65 and VCAM-1 expression of different groups. Quantitation of p-p65 level (D) and VCAM-1 level relative to ß-actin (E). SP SP600125; si-Neg was control for si-JNK. *P < 0.05 versus static group. ●P < 0.05 versus control within low group.