Figure 2

Effect of plasma from NSCLC patients or AGE-modified FCS on the growth of NSCLC cells in vitro. (A) H358 spheroids were treated with the individual plasma of randomly selected NSCLC patients. The spheroid growth after 2 d was plotted per AGE-related fluorescence (FL) of the individual plasma sample and subjected to linear regression analysis. (B) The proliferation and migration of H358 cells (cell proliferation and wound scratch assay) as well as (C) the spheroid growth of H358 cells was analyzed after 2 d in response to AGE-modified versus control (unmodified) FCS. Mean data ± SD are given. P values were calculated by the Student t test.