Figure 10

Comparison of Ca²⁺ mobilization by AAT in Ca²⁺−free versus Ca²⁺− containing buffer. Neutrophils loaded with the fluorescent Ca²⁺ indicator Fluo-3 were measured flow cytometrically in a buffer containing 1 mmol/L Ca²⁺ (A, C) or Ca²⁺− free buffer containing 1 mmol/L EGTA (B, D). Measurement was as described in Materials and Methods and in Figure 4. The black curves indicate the median fluorescence of neutrophils over time. The arrows indicate addition of 1 mg/mL (A, B) or 2 mg/mL (C, D) AAT.