Figure 12
From: Acute-Phase Protein α1-Antitrypsin Inhibits Neutrophil Calpain I and Induces Random Migration
AAT inhibits LPS-induced IL-8 release from neutrophils. Neutrophils were re-suspended in RPMI 1640 medium at a concentration of 3 × 106 cells/mL and stimulated with LPS (10 ng/mL), AAT (0.5 mg/mL) alone and LPS/AAT together for 4 h at 37°C, 5% CO2. Supernatants of cell cultures were analyzed to determine the levels of IL-8. Each bar represents the mean ± SD from four independent experiments.