Figure 13

AAT does not decrease CD16 expression (A) and does not induce neutrophil apoptosis (B). Neutrophils were treated with AAT (0.5 mg/mL) for 4 h at 37°C, 5% CO₂. (A) Cell lysates from 3 × 10⁶ neutrophils were separated on 7.5% SDS-PAGE, followed by immunoblotting, as described in Materials and Methods. Immunoblotting was performed using a monoclonal antibody against human CD16. The results shown are representative of two independent experiments. (B) Neutrophil apoptosis was monitored using an Annexin V-FITC apoptosis detection kit (BD Biosciences Pharmingen™). Cells were analyzed by flow cytometry. Unstained cells, and cells stained with FITC Annexin V or PI, were used controls.