Effects of AAT on neutrophil adhesion to fibronectin. Calcein-AM-labeled neutrophils were added to fibronectin-coated 96-well plates and stimulated with AAT, fMLP (100 nmol/L), fMLP/AAT or medium alone, as described in Materials and Methods. Adherent cells were analyzed with a fluorescence spectrophotometer using an excitation λ of 485 nm and emission λ of 520 nm. The results are expressed as a percentage of neutrophil adhesion ([adherent cells/total cells] × 100%). Each bar represents the mean ± SD of three independent experiments, each performed in four repeats.