Neutrophil random migration induced by AAT. The videos were recorded with the temporal resolution of 5 s using a Hamamatsu ORCA-R2 CCD camera. Cell migration rates were determined using the Time Lapse Analyzer (TLA) software. Tracks of the cells that remained round after stimulation were manually excluded from the analysis. Values are expressed as mean ± SD, with track numbers n = 35 for control cells and n = 119 for AAT stimulated cells. In the presence of AAT, cell migration was significantly enhanced compared with nontreated controls.