Figure 4

Time-dependent changes induced by hypoxia in a mouse model. Mice were housed in either normoxia (control) or 10% normobaric hypoxia for 3, 10 or 42 d. (A) Right ventricular systolic pressure measured during 100% oxygen inhalation increased at 10 and 42 d (25.24 ± 4.57 and 23.56 ± 4.46 mmHg, respectively), compared with normoxia controls (15.10 ± 2.91 mmHg); (B) hypoxia significantly increased RV/S + LV ratios (normoxia controls; 0.2563 ± 0.0425, hypoxia: 10 d: 0.3945 ± 0.039 and 42 d: 0.4101 ± 0.0276); (C) MIF mRNA increased in the lung tissue from d 3 (d 3, 10 and 42: 7.6-, 15.6-, and 1.9-fold increases, respectively); (D) plasma MIF protein concentrations were increased at d 10 and 42 d of hypoxia (2.01- and 1.58-fold increases respectively versus normoxia). n = 10 in normoxia and hypoxia 42-d group, n = 5 in hypoxia 3-d and 10-d group, *P < 0.05; **P < 0.01 versus normoxia.