cAng-1 improved vascular integrity by regulating the membrane localization of VE-cadherin. (A) FITC-dextran permeability of endothelial monolayer. Endothelial cells seeded on transwell filters were incubated under hypoxic conditions for 16 h and then reoxygenated for 2 h (H/Reoxy). Tie-2Fc (10 µg/mL) was added 30 min before cAng-1 (200 ng/mL) treatment. n = 3, *P < 0.05. The effect of cAng-1 was blocked by Tie-2Fc. (B) H/Reoxy-induced shift of VE-cadherin from membrane to cytosol fraction, which was prevented by cAng-1 treatment. Distribution of VE-cadherin at the membrane and in the cytosol fraction is shown (top). The quantification graph of VE-cadherin immunoblots (bottom) is shown (n = 3; *P < 0.01; **P< 0.05; Tie-2Fc [10 µg/mL); cAng-1 (200 ng/mL)).