cAng-1 promoted cardiomyocyte survival under H/Reoxy conditions via integrin-β1. Each experiment showed rat neonatal cardiomyocyte viability after 16 h of hypoxia and 2 h of reoxygenation (H/Reoxy). IgG, anti-integrin-β1 and anti-integrin-β3 (50 µg/mL) were added 30 min before cAng-1 (200 ng/mL). Blocking antibody against integrin-β1 completely abolished the protective effect of cAng-1 on cardiomyocytes after H/Reoxy. (A) Lactate dehydrogenase (LDH) analysis (n = 3, *P< 0.05; P = NS, no significance). (B) PI-fluorescence-activated cell sorter (FACS) analysis (n = 3, *P < 0.05). (C) Immunofluorescence staining for cleaved caspase-3 and TUNEL. Arrows, caspase-3+/TUNEL+ cells; arrowheads, caspase-3+ cells. Scale bar = 40 µm. (D) TUNEL+ cells were counted in 10 different microscopic fields in at least 3 different culture plates (*P < 0.01).