Figure 5
cAng-1 suppressed apoptotic machinery in cardiomyocytes under H/Reoxy by an integrin-β1/ERK-dependent manner. Neonatal cardiomyocytes were incubated for 16 h of hypoxia and 2 h of reoxygenation (H/Reoxy). (A, B) Representative immunoblot and quantitative graph of cleaved caspase-3, Bcl-XL and cleaved caspase-9 (n = 4, each). Integrin-β1 blockade completely reversed the protective effect of cAng-1. (C) Levels of phosphorylated Akt, GSK-3β(Ser9) and ERK. Integrin-β1 neutralizing remarkably blocked the phospho-ERK. (D) PD98059 (10 μmol/L) or LY294002 (10 μmol/L) was incubated for 30 min before exposure to cAng-1. Only PD98059, an inhibitor of the ERK-MAPK pathway, blocked caspase-9 suppression by cAng-1.