Transcriptome analysis revealed that MACs represent angiogenic M2-activated macrophages. (A) Heat maps of typical M1, M2 markers and proangiogenic genes demonstrated that the MAC gene signature is highly enriched for M2 markers, whereas expression of Ml macrophage markers was weak/low. Proangiogenic genes were also highly expressed. (B) Gene expression profiles of MACs were normalized to monocytes. Proangiogenic genes (green) and M2 markers (purple) were upregulated and M1 markers (red) were downregulated compared with housekeeping genes (blue).