Skip to main content
Figure 7 | Molecular Medicine

Figure 7

From: Myeloid Angiogenic Cells Act as Alternative M2 Macrophages and Modulate Angiogenesis through Interleukin-8

Figure 7

IL-8 transactivates VEGFR2 and induces ERK phosphorylation in endothelial cells. (A) HMEC-1 cells were treated with 100 ng/mL IL-8 for 15 min, followed by immunoblot analysis using a phosphoVEGFR2 antibody. IL-8 increased VEGFR2 phosphorylation levels compared with controls; 100 ng/mL VEGF treatment was used as a positive control. (B) RMECs were also treated with 100 ng/mL IL-8 for 15 min, and immunoblot analysis for phospho-ERKs was performed. IL-8 induced ERK phosphorylation in RMECs; this effect was completely abolished by the CXCR2 inhibitor SB225002.

Back to article page