AEBP1 promotes diet-induced hyperlipidemia and atherosclerosis in mice. (A) Whole blood samples were obtained from 32-wk-old, HFD-fed AEBP1TG and AEBP1NT mice to measure total cholesterol and triglyceride serum levels by gas chromatograph. Concentration (Conc.) of total cholesterol and triglycerides is expressed as mg/dL, and data are demonstrated as mean ± SEM (n = 3–5). Statistical significance was determined relative to cholesterol or triglyceride serum level in AEBP1NT mice for each gender. To assess lipid-filled lesion formation, aortic cryosections obtained from 12- and 32-wk-old, chow- and HFD-fed AEBP1TG and AEBP1NT mice were stained with ORO. Representative images of ORO-stained aortic cryosections from 32-wk-old, HFD-fed AEBP1TG (B, D) and AEBP1NT (C, E) females are shown at 100× (B, C) and 400× (D, E) magnification. A total of 3–35 mice per group were examined (refer to Table 1 for more details).