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Figure 2 | Molecular Medicine

Figure 2

From: Adipocyte Enhancer-Binding Protein 1 (AEBP1) (a Novel Macrophage Proinflammatory Mediator) Overexpression Promotes and Ablation Attenuates Atherosclerosis in ApoE−/− and LDLR−/− Mice

Figure 2

Ablation of AEBP1 attenuates atherosclerosis in LDLR-null mice. (A) En face staining of aortas of AEBP1+/+/LDLR−/− and AEBP1−/−/LDLR−/− male and female mice. Mice were fed an atherogenic diet for 13 wks, and aortic lesion was analyzed en face with sudan IV staining. (B) Graphs showing quantification of atherosclerotic lesion size as a percentage of total aortic area. Error bars represent SEM, **P ≤ 0.001 (n = 5 males, P = 0.001), *P ≤ 0.05 (n = 5 females, P = 0.0026). (C) Cross-sectional analysis at the aortic root of samples isolated from AEBP1+/+/LDLR−/− and AEBP1−/−/LDLR−/− mice. Representative ORO staining in the aortic sinus after 13 wks on the atherogenic diet. (D) Graphs showing quantification of atherosclerotic lesion size at the aortic sinus as a percentage of total aortic root area. Error bars represent SEM, **P < 0.001 (n = 5 males, P = 0.0004), *P < 0.05 (n = 5 females, P = 0.0126). (E) Immunohistochemical analysis of the aortic atherosclerotic lesion (females) at low magnification (40×). Tissue sections obtained from aortic atherosclerotic lesion were treated with normal rat IgG, rat anti-mouse F4/80 (macrophage), rat anti-mouse CD106 (VCAM-1) and rat anti-mouse CD3 (T cells).

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