Atherogenesis is significantly accelerated in ApoE−/−/TG-BM mice compared with ApoE−/−/NT-BM mice. (A) Experimental design of generation of radiation chimera and list of chimeras (n = 5) for each chimera. (B) En face analysis of aorta atherosclerotic lesion formation in ApoE−/−/NT-BM and ApoE−/−/TG-BM chimeras. Lipid staining was performed using sudan IV reagent. Quantification of atherosclerotic lesion area was performed with ImageJ; numbers represent the atherosclerotic lesion as a percentage of total aorta area (***P = 0.0001). (C) ORO staining of atherosclerotic lesions of aortic roots in ApoE−/−/NT-BM and ApoE−/−/TG-BM chimeras. Graphs showing quantification of atherosclerotic lesion size at the aortic sinus as a percentage of total aortic root area (***P = 0.0001). (D) Analysis of total plasma cholesterol and triglyceride levels in ApoE−/−/NT-BM and ApoE−/−/TG-BM chimeras.