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Figure 6 | Molecular Medicine

Figure 6

From: Adipocyte Enhancer-Binding Protein 1 (AEBP1) (a Novel Macrophage Proinflammatory Mediator) Overexpression Promotes and Ablation Attenuates Atherosclerosis in ApoE−/− and LDLR−/− Mice

Figure 6

PPARγ1 and LXRα expression and proinflammatory mediators are significantly up-regulated in ApoE−/−/TG-BM macrophages compared with ApoE−/−/NT-BM macrophages. (A) Relative protein levels of PPARγ1 and LXRα in macrophages from chimeras were analyzed by immunoblotting with specific antibodies. (B) Densitometric analysis on the basis of γ-actin expression in macrophage from chimeras is shown. (C) Transcript levels of PPARγ1, ApoE, IL-6 and TNF-α were assessed by real-time PCR analysis. Statistical significance was determined relative to protein expression level or mRNA level in macrophages isolated from ApoE−/−/NT-BM mice (n = 5). Transcript levels of ABCA1 and ABCG1 were assessed by real-time PCR analysis in triplicate with pooled samples (n = 5). ***P< 0.001.

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Molecular Medicine

ISSN: 1528-3658