Production of hAAT in mice by HD injection of pEF-hAAT. Expression of hAAT in mouse liver: Heterozygote hAAT transgenic mice were administered either PBS (HD-PBS, n = 3) or pEF-hAAT (HD-pEF-hAAT, 100 µg, n = 3) via HD tail-vein injection (1.8 mL/6 s). (A) Twenty-four hours later, animals were sacrificed and liver samples were examined for hAAT expression by RT-PCR of extracted liver RNA using primers specific for hAAT (top). Western blot analysis of extracted liver protein using an anti-hAAT antibody is shown (bottom). Protein size and specificity are compared to clinical-grade hAAT (Aralast™). (B) Immunohistochemistry (blue, nuclear DAPI stain; red, hAAT antibody).