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Table 1 Effect of RSVA314 and RSVA405 treatment on adipogenesis-induced gene expression changes in 3T3-L1 cells.

From: Small-Molecule Activators of AMP-Activated Protein Kinase (AMPK), RSVA314 and RSVA405, Inhibit Adipogenesis

Gene

Differentiated

RSVA314

RSVA405

Ceacam1

3.04 ± 0.37a

0.60 ± 0.12b

0.81 ± 0.26b

Trib3

0.08 ± 0.03a

0.72 ± 0.14b

0.69 ± 0.08b

PPARg

1.57 ± 0.05c

0.72 ± 0.04b

0.67 ± 0.06b

Agt

5.46 ± 2.17c

0.36 ± 0.17b

0.36 ± 0.12b

Pik3r1

1.59 ± 0.12c

0.87 ± 0.07b

0.84 ± 0.06b

Cebpa

2.79 ± 0.21c

0.70 ± 0.07d

0.74 ± 0.06d

Retn (resistin)

4.95 ± 0.45c

1.12 ± 0.05d

1.24 ± 0.22d

Slc2a4 (GLUT4)

11.20 ± 7.41c

2.48 ± 1.55d

1.47 ± 0.28d

Snap25

4.94 ± 1.23c

1.11 ± 0.04d

1.23 ± 0.21d

Srebf1

1.79 ± 0.04c

0.96 ± 0.08d

0.91 ± 0.05d

Foxc2

1.94 ± 0.06c

1.13 ± 0.16d

1.13 ± 0.10d

Ccl5 (RANTES)

0.54 ± 0.07e

1.12 ± 0.17b

1.74 ± 0.20b

Gpd1

1.67 ± 0.31e

0.78 ± 0.16b

0.39 ± 0.18b

Vamp2

0.76 ± 0.09e

1.42 ± 0.13b

1.18 ± 0.19d

Pfkfb3

2.45 ± 0.91e

1.04 ± 0.51d

1.33 ± 0.45d

II4ra

1.53 ± 0.01e

1.03 ± 0.07f

0.75 ± 0.09d

Igfbp5

3.73 ± 0.87e

1.12 ± 0.05f

1.24 ± 0.22f

Nrf1

0.73 ± 0.05e

0.97 ± 0.03f

0.96 ± 0.03f

Ace

2.49 ± 0.46e

1.87 ± 0.51

1.24 ± 0.22f

Icam1

0.31 ± 0.13

2.03 ± 0.51

2.23 ± 0.35f

  1. Gene expression was analyzed by real-time PCR using RNA from 3T3-L1 cells differentiated for 2 d and treated or not with the RSVAs (2 μmol/L). Data are expressed as fold-change compared with nondifferentiated control condition ± standard deviation (SD) of three independent experiments.
  2. aP < 0.001, cP < 0.01, eP < 0.05, versus nondifferentiated condition.
  3. bP < 0.001, dP < 0.01, fP < 0.05, versus differentiated condition (Student ttest).