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Table 1 Effect of RSVA314 and RSVA405 treatment on adipogenesis-induced gene expression changes in 3T3-L1 cells.

From: Small-Molecule Activators of AMP-Activated Protein Kinase (AMPK), RSVA314 and RSVA405, Inhibit Adipogenesis

Gene Differentiated RSVA314 RSVA405
Ceacam1 3.04 ± 0.37a 0.60 ± 0.12b 0.81 ± 0.26b
Trib3 0.08 ± 0.03a 0.72 ± 0.14b 0.69 ± 0.08b
PPARg 1.57 ± 0.05c 0.72 ± 0.04b 0.67 ± 0.06b
Agt 5.46 ± 2.17c 0.36 ± 0.17b 0.36 ± 0.12b
Pik3r1 1.59 ± 0.12c 0.87 ± 0.07b 0.84 ± 0.06b
Cebpa 2.79 ± 0.21c 0.70 ± 0.07d 0.74 ± 0.06d
Retn (resistin) 4.95 ± 0.45c 1.12 ± 0.05d 1.24 ± 0.22d
Slc2a4 (GLUT4) 11.20 ± 7.41c 2.48 ± 1.55d 1.47 ± 0.28d
Snap25 4.94 ± 1.23c 1.11 ± 0.04d 1.23 ± 0.21d
Srebf1 1.79 ± 0.04c 0.96 ± 0.08d 0.91 ± 0.05d
Foxc2 1.94 ± 0.06c 1.13 ± 0.16d 1.13 ± 0.10d
Ccl5 (RANTES) 0.54 ± 0.07e 1.12 ± 0.17b 1.74 ± 0.20b
Gpd1 1.67 ± 0.31e 0.78 ± 0.16b 0.39 ± 0.18b
Vamp2 0.76 ± 0.09e 1.42 ± 0.13b 1.18 ± 0.19d
Pfkfb3 2.45 ± 0.91e 1.04 ± 0.51d 1.33 ± 0.45d
II4ra 1.53 ± 0.01e 1.03 ± 0.07f 0.75 ± 0.09d
Igfbp5 3.73 ± 0.87e 1.12 ± 0.05f 1.24 ± 0.22f
Nrf1 0.73 ± 0.05e 0.97 ± 0.03f 0.96 ± 0.03f
Ace 2.49 ± 0.46e 1.87 ± 0.51 1.24 ± 0.22f
Icam1 0.31 ± 0.13 2.03 ± 0.51 2.23 ± 0.35f
  1. Gene expression was analyzed by real-time PCR using RNA from 3T3-L1 cells differentiated for 2 d and treated or not with the RSVAs (2 μmol/L). Data are expressed as fold-change compared with nondifferentiated control condition ± standard deviation (SD) of three independent experiments.
  2. aP < 0.001, cP < 0.01, eP < 0.05, versus nondifferentiated condition.
  3. bP < 0.001, dP < 0.01, fP < 0.05, versus differentiated condition (Student ttest).