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Figure 1 | Molecular Medicine

Figure 1

From: The p53 Upregulated Modulator of Apoptosis (PUMA) Chemosensitizes Intrinsically Resistant Ovarian Cancer Cells to Cisplatin by Lowering the Threshold Set by Bcl-xL and Mcl-1

Figure 1

p53-dependent activation of PUMA by cisplatin in ovarian cancer cells. (A) A2780s (p53 WT) and SKOV3 (p53−/−) cells were treated with cisplatin (5 µg/mL) for 24 h and analyzed for the expression of p53 and PUMA by Western blotting. GAPDH was used as a loading control. Densitometric analysis of the Western blots was performed, and the amount of p53 and PUMA was compared with the protein loading control. The relative amount of p53 and PUMA from the untreated A2780s cells was set as 1. (B) Semiquantitative RT-PCR was done for PUMA, p53 and the control GAPDH (25 cycles) using the cDNA prepared from cells with or without cisplatin treatment.

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