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Figure 3 | Molecular Medicine

Figure 3

From: The p53 Upregulated Modulator of Apoptosis (PUMA) Chemosensitizes Intrinsically Resistant Ovarian Cancer Cells to Cisplatin by Lowering the Threshold Set by Bcl-xL and Mcl-1

Figure 3

Induction of apoptosis of tumor cells in vitro by hPUMA and cisplatin. (A) Representative DNA fluorescence histograms of propidium iodide (PI)-stained cells. A2780s cells were treated with hPUMA for 24 h and then with 5 µg/mL cisplatin for an additional 24 h. A2780s cells were untreated or treated with pcDNA3.1 or hPUMA, cisplatin alone or hPUMA plus cisplatin, and groups Ctrl, pc3.1, hPUMA, Cis (cisplatin) and hPUMA + Cis correspond to these five treatments (the same as shown in the subsequent panels), with 10.1% (Ctrl), 19.3% (pc3.1), 40.3% (hPUMA), 49.3% (Cis) and 62.4% (hPUMA + Cis) sub-G1 cells (apoptotic cells), respectively, as assessed by flow cytometry. (B) SKOV3 cells were treated with hPUMA for 24 h and then with 5 µg/mL cisplatin for an additional 24 h. SKOV3 cells were untreated, treated with empty vector or hPUMA, cisplatin alone or hPUMA plus cisplatin, with 9.4% (Ctrl), 11.1% (pc3.1), 41% (hPUMA), 12.5% (Cis) and 53.5% (hPUMA + Cis) sub-G1 cells (apoptotic cells), respectively, as assessed by flow cytometry. (C) Normal and apoptotic nuclear morphology of A2780s cells was analyzed by Hoechst 33258 staining. A2780s cells were treated with the same conditions as described above. (D) Normal and apoptotic nuclear morphology of SKOV3 cells was analyzed by Hoechst 33258 staining. SKOV3 cells were treated with the same conditions as mentioned above.

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