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Figure 8 | Molecular Medicine

Figure 8

From: The p53 Upregulated Modulator of Apoptosis (PUMA) Chemosensitizes Intrinsically Resistant Ovarian Cancer Cells to Cisplatin by Lowering the Threshold Set by Bcl-xL and Mcl-1

Figure 8

Detection of hPUMA overexpression in vivo. (A) The A2780s tumor-bearing mice (five mice per group) were treated with PBS or liposome-pGL3-control luciferase reporter plasmid complex via the tail vein twice a week for 4 wks. Lysates of tumors were obtained, and luciferase values were read on a luminometer. Luciferase activities 48 h after the last injection of A2780s tumors treated with PBS or pGL3-control expression plasmid were shown in the graph. Statistically significant difference compared with the PBS treatment group (**P < 0.001) is shown. (B) RT-PCR analysis of exogenous hPUMA expression in A2780s tumor tissues. (C) RT-PCR analysis of exogenous hPUMA expression in SKOV3 tumor tissues. Total RNA samples were isolated from A2780s and SKOV3 tumor tissues in the untreated group, pcDNA3.1 treatment group and pcDNA3.1-hPUMA treatment group, respectively.

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Molecular Medicine

ISSN: 1528-3658