Figure 3

Elevated plasma SAA level induces the accumulation of macrophages in atherosclerotic regions of chow-fed mice. (A) Representative images by immunohistochemistry staining for macrophages (brown) (bar = 100 µm). *P< 0.01 compared with the lenti-null group (n = 10 for both groups). (B) The colocalization of SAA (red) with macrophages (green) by immunofluorescence analysis (Magnification 100×). (C) Migration assay of chemotaxis of SAA to macrophages by dilution dose of SAA with DMEM. Data are mean ± SD from 5 separate fields in each sample from 3 independent experiments (bar = 50 µm). *P < 0.05 versus control group and #P < 0.05 versus 1:50 group.