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Figure 4 | Molecular Medicine

Figure 4

From: Microglial Stimulation of Glioblastoma Invasion Involves Epidermal Growth Factor Receptor (EGFR) and Colony Stimulating Factor 1 Receptor (CSF-1R) Signaling

Figure 4

Glioblastoma and microglia cells express CSF-1R and EGFR ligands. (A) Relative CSF-1 concentrations in supernatants from GL261 cells cultured in serum-free media with 0.3% BSA alone (open bar) or with microglia (closed bar; MG), 10 nmol/L EGF (closed bar; EGF), DMSO or 1 µmol/L Iressa overnight were determined by ELISA. Results are normalized to GL261 alone and are the means ± SEM of three independent experiments. (B) Western blot of microglia extracts using an anti-EGF antibody. Blot is representative of four experiments. (C) Immunofluorescence of paraformaldehyde-fixed microglia using an anti-EGF antibody. Image is representative of four independent experiments. Scale bar = 50 µm. A.U., arbitrary units.

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