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Figure 5 | Molecular Medicine

Figure 5

From: Microglial Stimulation of Glioblastoma Invasion Involves Epidermal Growth Factor Receptor (EGFR) and Colony Stimulating Factor 1 Receptor (CSF-1R) Signaling

Figure 5

Glioblastoma and microglia respond to EGF and CSF-1, respectively. (A) Glioblastoma cells respond to EGF but not CSF-1R. GL261 cells (100,000) were plated on transwell chambers lightly coated with Matrigel, and migration in response to buffer (open bar), 50 ng/mL CSF-1 (closed bar; +CSF1), or 10 nmol/L EGF (closed bar; +EGF) in the bottom well was measured. Transwells were fixed and stained with crystal violet, and the extent of migration relative to buffer was determined by counting the cells on the underside of the filter. Means ± SEM of data from three experiments. *P < 0.01 compared with buffer control. (B) Western blot of GL261 and microglia extracts using an anti-CSF-1R antibody. Blot is representative of three experiments. (C) Microglia are induced to invade by CSF-1 but not EGF. Microglia (100,000) were cultured on Matrigel-coated transwells, and invasion in response to buffer (open bar), 50 ng/mL CSF-1 (closed bar; +CSF1) or 10 nmol/L EGF (closed bar; +EGF) in the bottom well was measured. Transwells were fixed and stained with crystal violet, and the extent of migration was determined by counting the cells on the underside of the filter. Means ± SEM of data from three experiments. *P < 0.01 compared with buffer control.

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