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Figure 6 | Molecular Medicine

Figure 6

From: Microglial Stimulation of Glioblastoma Invasion Involves Epidermal Growth Factor Receptor (EGFR) and Colony Stimulating Factor 1 Receptor (CSF-1R) Signaling

Figure 6

Blockade of CSF-1R in vivo inhibits microglia/macrophage recruitment to GL261 tumors. (A) GL261 cells (2 × 104) were intracranially injected into 8- to 12-wk-old C57BL/6 mice and allowed to recover for 24 h. Animals were then split into two groups: one fed control chow (CTL) and one fed with chow containing PLX3397. After 2 wks, animals were sacrificed and brains were fixed in formalin solution and paraffin embedded followed by immunohistochemistry staining using Iba1. Representative images were taken with a 10× objective. Scale bar = 100 µm. (B) The degree of microglia/macrophage infiltration in tumors isolated from animals fed with control chow (open bar) or PLX3397 chow (closed bar) was quantified by counting the number of Iba1+ cells per mm2 of tumor. Results shown are means ± SEM of data of at least eight tumors. *P < 0.001 compared with control.

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