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Figure 3 | Molecular Medicine

Figure 3

From: Prime-Boost Vaccination with rBCG/rAd35 Enhances CD8+ Cytolytic T-Cell Responses in Lesions from Mycobacterium Tuberculosis-Infected Primates

Figure 3

Vaccination with rBCG/rAd35 resulted in elevated levels of different lymphocyte populations in lung and spleen from Mtb-infected animals. (A) Microscopic images of CD20+ B cells (upper panel) and CD3+ (middle panel) and CD8α T cells (lower panel) in TB lung lesions from the rBCG/rAd35 and BCG/rAd35 vaccine groups as well as the unvaccinated control. Immunohistochemical staining was performed, and granulomatous areas (gr) with the presence of characteristic giant cells (GC; arrowheads) are shown in the TB lesions. Arrows indicate positive cells and cell infiltrates (brown), whereas negative cells (blue) are counterstained with hematoxylin. Magnification 125×. Protein expression of CD20+ B cells (B), CD3+ T cells (C), CD8α+ T cells (D), CD8ÎČ+ T cells (E), and CD4+ T cells (F) was assessed using computerized image analysis to determine the median protein expression (± IQR) in lung lesions and spleen tissues among rBCG (rBCG/rAd35) or BCG (BCG/rAd35) vaccinated and control (unvaccinated) animals. The data from n = 5–6 animals/group are presented as percent positive area of the total cell area. Statistical significance of differences in protein expression was determined by a nonparametric Kruskal-Wallis test. *P < 0.05, **P < 0.01.

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