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Figure 1 | Molecular Medicine

Figure 1

From: p38 Mitogen-Activated Protein Kinase and Liver X Receptor-α Mediate the Leptin Effect on Sterol Regulatory Element Binding Protein-1c Expression in Hepatic Stellate Cells

Figure 1

Leptin reduces SREBP-1c gene expression in HSCs in vitro and in vivo. (A-C) Western blot and real-time PCR analysis of the protein and mRNA levels of SREBP-1c, respectively (n = 3). HSCs were stimulated with leptin at the indicated doses for different periods of time (A) or 24 h (B, C). *P < 0.05 versus cells without leptin. (D) Transfection assay for analysis of SREBP-1c promoter activity. HSCs in 12-well plastic plates were transfected with pSREBP1c-Luc and treated with or without 100 ng/mL leptin for 24 h (n = 6). *P < 0.05 versus cells without leptin. (E) Immunofluorescence staining of liver sections for SREBP-1c in HSCs. C57BL/6J ob/ob mice and lean littermates were received TAA (200 µg/g body weight, two times a week) or TAA plus leptin (leptin, 1 µg/g body weight) for 4 wks. Double immunofluorescence staining of liver sections for synaptophysin (SYN, red fluorescence) and SREBP-1c (green fluorescence) were performed (n = 6). The nuclei were counter-stained with Hoechst 33342 (blue fluorescence) (original magnification, 200×).

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