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Table 2 Primers for the B cell receptor heavy chain immunoscope analysis.

From: Preserving the B-Cell Compartment Favors Operational Tolerance in Human Renal Transplantation

Primer Sequence
IgVH family  
VH1 5′-TGGAGCTGAGSAGSCTGAGATCYGA-3′
VH2 5′-AACCCACASAGACCCTCAC-3′
VH3 5′-TCCCTKARACTCTCCTGTRCAGC-3′
VH4 5′-CTACAACCCSTCCCTCAAGAGT-3′
VH5 5′-CAGCACCGCCTACCTGCAGTGGAGC-3′
VH6 5′-TCCGGGGACAGTGTCTCT-3′
VH7 5′-CAGCACRGCATAYCTGCAGATCAG-3′
IgH γ chain 5′-6Fam-AAGTAGTCCTTGACCAGGCAGC-3′
IgH µ chain 5′-6Fam-GGAGACGAGGGGGAAAAGG-3′
  1. The primers used to perform PCR in the immunoscope analysis of the 7 VH immunoglobulin families (VH1–VH7) for the IgG and IgM isotypes are shown.