Figure 6

NO/iNOS mimics the effects seen in IRF-1 overexpression on autophagy in RAW264.7 cells. (A) Raw cells were pretreated with serum-free media for 12 h and then stimulated with a different dose of SNAP, an NO donor for 16 h. Cells were harvested for western blot analysis for p-mTOR, p-P70S6, LC3B I/II and P62. *P < 0.05; Results representative of three separate independent experiments. (B) RAW 264.7 cells were pretreated with L-NIL (200 µmol/L) for 1h and then stimulated with LPS (500 ng/mL) for 16 h. Cells were harvested for Western blot analysis for LC3B and p62. *P < 0.05; results representative of three separate independent experiments.