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Figure 1 | Molecular Medicine

Figure 1

From: Oxidized Low-Density Lipoprotein-Dependent Platelet-Derived Microvesicles Trigger Procoagulant Effects and Amplify Oxidative Stress

Figure 1

Detection of PMVs by flow cytometry and ELISA. A–E, Flow cytometry detection of platelets and PMVs. Platelets were incubated with buffer control (A), 50 µg/mL native low-density lipoprotein (nLDL) (B), 50 µg/mL oxLDL (C) or 10 µmol/L ADP (D) for 15 min at 37°C. P and M indicate the gates for platelets and PMVs, respectively (E). (F–G) MFI of CD41a in M gates, ELISA quantification of total PMVs (F) and annexin V-positive PMVs (G). Platelets were incubated with 50 µg/mL oxLDL or nLDL, or 10 µmol/L ADP Data are means ± SE from three independent experiments. *P < 0.05 compared with control.

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