Figure 1From: Oxidized Low-Density Lipoprotein-Dependent Platelet-Derived Microvesicles Trigger Procoagulant Effects and Amplify Oxidative StressDetection of PMVs by flow cytometry and ELISA. A–E, Flow cytometry detection of platelets and PMVs. Platelets were incubated with buffer control (A), 50 µg/mL native low-density lipoprotein (nLDL) (B), 50 µg/mL oxLDL (C) or 10 µmol/L ADP (D) for 15 min at 37°C. P and M indicate the gates for platelets and PMVs, respectively (E). (F–G) MFI of CD41a in M gates, ELISA quantification of total PMVs (F) and annexin V-positive PMVs (G). Platelets were incubated with 50 µg/mL oxLDL or nLDL, or 10 µmol/L ADP Data are means ± SE from three independent experiments. *P < 0.05 compared with control.Back to article page