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Figure 6 | Molecular Medicine

Figure 6

From: Oxidized Low-Density Lipoprotein-Dependent Platelet-Derived Microvesicles Trigger Procoagulant Effects and Amplify Oxidative Stress

Figure 6

PMVs induced phosphorylation of JNK2 and its upstream activator MKK4 in CD36-positive platelets. CD36-positive and CD36-deficient platelets were incubated with PMVs and then lysed. Phosphorylated forms of JNK1/2 and MKK4 were detected by Western blot analysis for phospho-MKK4 (p-MKK4) and phospho-JNK (p-JNK1/2) and total MKK4 and JNK. (A) PMVs induced activation of JNK2 in CD36-positive but not CD36-deficient platelets. CD36-positive platelets were incubated with PMVs (30 µg/mL) for various times (B) and various concentrations of PMVs (C) for detection of phosphorylation of JNK2. Data are means ± SE of three independent experiments from different donors; *P < 0.05. □, Control; ■, PMVs.

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