Skip to main content
Figure 10 | Molecular Medicine

Figure 10

From: Hematopoietic CC-Chemokine Receptor 2 (CCR2) Competent Cells Are Protective for the Cognitive Impairments and Amyloid Pathology in a Transgenic Mouse Model of Alzheimer’s Disease

Figure 10

Treatment with lentivirus expressing the CCR2 gene in the bone marrow induces CCR2 expression on the surface of Ly6-C+ monocytes in CCR2−/− mice. Five-month-old CCR2−/− mice received intrafemoral injections of a lentiviral construction containing either GFP or GFP with a CCR2 transgene. Four weeks later, whole blood was stained with fluorescent antibody for CD11b, CD115, Ly6-C and CCR2 and analyzed by FACS. Monocytes were characterized by CD11b and CD115 expression in CCR2−/− mice injected with lenti-GFP (A) or lenti-GFP-CCR2 (B). In monocytes, Ly6-C and CCR2 expression was assessed in CCR2−/− mice injected with lenti-GFP (C) or lenti-GFP-CCR2 (D). Histograms (E, F) represent CCR2 signal in CD11b+CD115+Ly6-C+CCR2+ cells. Each graph shows a representative example of CCR2−/− mice injected with lenti-GFP (left column) or lenti-GFP-CCR2 (right column). Numbers near boxes indicate the percentage of CD11b+ CD115+ cells (A, B) or CD11b+ CD115+ Ly6-C+ CCR2+ cells (C, D) for this example. The percentage of CCR2+ cells was determined in leukocytes (G) and monocytes (H). Interestingly, lenti-GFP-CCR2-treated mice exhibited CCR2 expression in leukocytes, which is significantly different in monocytes from control lenti-GFP-treated mice. Results are expressed as the mean ± SEM; n = 2−3; Student t test; *P < 0.05 versus lenti-GFP injected mice.

Back to article page