Figure 11

Treatment with lentivirus expressing a CCR2 transgene in the bone marrow increases the frequency of monocytes, especially in circulating Ly6-C⁺, Gr1⁺ and Gr1⁻ subsets of CCR2^−/− mice. Five-month-old CCR2^−/− mice received intrafemoral injections of a lentiviral construction containing GFP or GFP with a CCR2 transgene. Three weeks later, CD11b, CD115, Ly6-C and Gr1 expression was analyzed by FACS in circulating leukocytes. Monocytes were characterized by CD11b and CD115 expression in CCR2^−/− mice injected with lenti-GFP (A) or lenti-GFP-CCR2 (B). In monocytes, Ly6-C and CD11b expression was assessed in CCR2^−/− mice injected with lenti-GFP (E) or lenti-GFP-CCR2 (F), as well as Gr1 expression (I and J, respectively). Intrafemoral injection of lenti-GFP-CCR2 increased the frequency of monocytes (CD11b⁺ CD115⁺ cells) (C, D), Ly6-C^high monocytes (G, H), as well as Gr1⁺ and Gr1⁻ monocytes (K, L). Each graph shows a representative example of CCR2^−/− mice injected with lenti-GFP (left column) or lenti-GFP-CCR2 (middle column). Numbers near boxes indicate the percentage of CD11b⁺ CD115⁺ cells (A, B), CD11b⁺ CD115⁺ Ly6-C^high cells (E, F), as well as CD11b⁺CD115⁺ Gr1⁺ or Gr1⁻ cells (I, J) for this example. Results are expressed as the mean ± SEM; n = 2−3; Student t test; *P < 0.05 versus lenti-GFP injected mice.