Figure 2

Aβ plaque formation remains similar in the brain of APP_Swe/PS1 and APP_Swe/PS1/CCR2^−/− mice transplanted with WT (GFP) or CCR2^−/− BMCs. Hemibrain sections of 6-month-old APP_Swe/PS1 and APP_Swe/PS1/CCR2^−/− mice transplanted with GFP or CCR2^−/− BMC (A–E) were immunostained for Aβ. Stereological analysis was performed to determine plaque density (F–G), plaque size (H–I) and the percentage area occupied by plaques (J–K) for each group in the hippocampus (F, H, J) and cerebral cortex (G, I, K). Transplantation of GFP or CCR2^−/− cells in APP_Swe/PS1 and APP_Swe/PS1/CCR2^−/− mice had no major effect on plaque formation. Results are expressed as the mean ± SEM; n = 6−15; *P < 0.05, **P < 0.01 and ***P < 0.001 versus APP_Swe/PS1; ^#P < 0.05 versus APP_Swe/PS1/CCR2^−/−. One-way ANOVA was performed using the Bonferroni or Tamhane post hoc test. Scale bar 500 µm. ■, APP_Swe/PS1; , GFP → APP_Swe/PS1;, CCR2^−/− → APP_Swe/PS1;, APP_Swe/PS1/CCR2^−/−; , GFP → APP_Swe/PS1/CCR2^−/−.