Figure 3
APPSwe/PS1 mice harboring WT (GFP) BMCs exhibit decreased levels of soluble extracellular and membrane-associated Aβ species, which are increased in APPSwe/PS1 mice after transplantation of CCR2−/− cells. Hemibrain extracts from soluble extracellular (A) and membrane-associated proteins (D) of 6-month-old APPSwe/PS1 mice harboring WT GFP or CCR2−/− cells in their bone marrow were assessed by Western blot on a 10–20% Tris-Tricine denaturing polyacrylamide gel to separate Aβ species using anti-Aβ antibody 6E10. The intensity of each band was quantified by densitometric analysis and normalized to β-actin values. Each Aβ species ratio (Aβ/β-actin) is represented for extracellular (B) and membrane-associated proteins (E). Lower levels of soluble extracellular and membrane-associated Aβ species were found in mice harboring WT BMCs, whereas Aβ oligomer levels increased in mice transplanted with CCR2-deficient BMCs. Correlations were determined between spatial memory decline (water T-maze test) and soluble Aβ species in extracellular (C) and membrane-associated (F) fractions. Results are expressed as the mean ± SEM; n = 4−5; *P < 0.05, **P < 0.01 and *** P < 0.001 versus APPSwe/PS1; #P < 0.05 versus GFP → APPSwe/PS1. One-way ANOVA was performed using Dunnett or Tamhane post hoc tests; for the Dunnett test, the control group was GFP → APPSwe/PS1 mice. Correlation was estimated by the Spearman correlation coefficient. OD, optical density; ■, APPSwe/PS1; 
, GFP → APPSwe/PS1; 
, CCR2−/− → APPSwe/PS1.