Figure 6
Expression of TGF-β1, and of TGF-β-R1 and -R2, mRNA in the brain of APPSwe/PS1 mice transplanted with WT (GFP) or CCR2-deficient BMCs. Representative dark-field photomicrographs of in situ hybridization are shown of the cortical expression of TGF-β1 (left panels), TGF-β-R1 (middle panels) and TGF-β-R2 mRNA (right panels) in 6-month-old APPSwe/PS1 and APPSwe/PS1/CCR2−/− harboring bone marrow GFP or CCR2−/− cells. Qualitative quantification was performed for each transcript (n = 5−12) (M). A decrease in TGF-β1, and in TGF-β-R1 and -R2, mRNA levels was found in the brain of mice transplanted with WT GFP cells. Scale bar 500 µm. M: The plus signs correspond to the intensity and the surface of the signal. The number of the plus signs shows augment proportionally with the intensity and the surface.