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Table 1 Plasmids and enzymes used for the synthesis of the cRNA probes.

From: Hematopoietic CC-Chemokine Receptor 2 (CCR2) Competent Cells Are Protective for the Cognitive Impairments and Amyloid Pathology in a Transgenic Mouse Model of Alzheimer’s Disease

Plasmid

Vector

Insert

Antisense probe

Sense probe

Source

Mouse CX3CR1

PCR II Topo

1132 bp

BamHI/T7

XhoI/Sp6

Cloned by PCR

Mouse MCP-1

pGEM-1

578 bp

BamHI/T7

SacI/SP6

SC Williams, Texas Tech University, Lubbock, TX, USA

Mouse TGFB1 R1

PCR II Topo

1093 bp

NotI/Sp6

SpeI/T7

Cloned by PCR

Mouse TGFB1 R2

PCR II Topo

933 bp

XhoI/Sp6

BamHI/T7

Cloned by PCR

Mouse TGFβ1

pBSKS+

1173 bp

EcoRI/T3

XhoI/T7

S Lacroix, Laval University, Québec, Canada