Figure 1

Flow cytometric analysis of SpA binding to primary B cells. (A) B cells were stained with CD20-PECy7 mAb, biot-Mod-SpA plus AF488-Streptavidin and IgD-PE mAb (left panels) or CD27-PE mAb (right panels). Data from one representative experiment of seven are shown. The percentages of positive cells gated on CD20⁺ cells are indicated in each quadrant. (B) B cells were incubated with medium, 10 µg/mL CD32 mAb or 10 ng/mL TNFα for 30 min at 4°C before staining with CD20-PEcy7 mAb and biot-Mod-SpA plus AF488-Streptavidin. Data from one representative experiment of 7 are shown. The percentages of Mod-SpA positive cells are indicated in the upper right quadrants.