Cochlear morphology of the Irs2+/+Ptpn1−/− mice. (A–D) Hematoxylineosin staining of midmodiolar paraffin sections of the cochlea (A, B) and cochlear ganglion (C, D) in wild-type and Irs2+/+Ptpn1−/− mice at postnatal wk 11. No gross differences were found. (E–H) Hematoxylin-eosin staining of the stria vascularis at the cochlear basal turn in wild-type (E, F) and Irs2+/+Ptpn1−/− (G, H) mice showed normal morphology, vasculature pattern and thickness in both genotypes. Details of the stria vascularis (F, H) illustrating the basal, intermediate and marginal cell layers and the normal capillary network are shown. (I, J) Kir4.1 immunoreactivity (red) revealed no evident differences between genotypes. Scale bars: A, B: 500 µm; C, D, E, G: 50 µm; F, H, I, J: 25 µm. B, basal cells; C, capillary network; I, intermediate cells; M, marginal cell.