Figure 5From: Milk Fat Globule-Epidermal Growth Factor 8 Is Decreased in Intestinal Epithelium of Ulcerative Colitis Patients and Thereby Causes Increased Apoptosis and Impaired Wound HealingValidation of MFG-E8 expression in IEC lines and MFG-E8 knockdown. (A) Western blot analysis of MFG-E8 protein level in HT-29 and Caco-2 cells. (B) Fluorescence microscopy of HT-29 and Caco-2 cells 72 h after transduction with copGFP Scale bar, 100 µm. (C) Real-time quantitative PCR analysis of MFG-E8 mRNA expression in LV-M- and LV-C-transduced HT-29 and Caco-2 cells. LV-M, lentiviral particles encoding short hairpin RNA sequences targeted to human MFG-E8 mRNA. LV-C, control lentiviral particles encoding a scrambled shRNA sequence. (D) Western blot analysis of MFG-E8 protein level in LV-M- and LV-C-transduced HT-29 and Caco-2 cells.Back to article page