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Figure 4 | Molecular Medicine

Figure 4

From: RETRACTED ARTICLE: Redox Modification of Cysteine Residues Regulates the Cytokine Activity of High Mobility Group Box-1 (HMGB1)

Figure 4

Effect of reducing agents on HMGB1-induced cytokine release. The (A) dose dependent and (B) time dependent effect of DTT on the TNF-stimulating activity of HMGB1 in RAW 264.7 cells. (C) The effect of DTT on the TNF-stimulating activity of LPS in RAW 264.7 cells. HMGB1 or LPS was incubated with the reducing agent DTT at (A) various concentrations as shown or with 5 mmol/L DTT at the times indicated at room temperature. Fresh DTT pretreated HMGB1 or LPS was then added to RAW 264.7 cells. After 16 h, TNF released into the cell culture supernatant was measured by ELISA. N = 3. *P < 0.05 versus HMGB1 alone without DTT pretreatment.

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Molecular Medicine

ISSN: 1528-3658