Figure 5

Deficiency of GNMT accelerates lipid accumulation in macrophage foam cells. A) Oil red O and hematoxylin staining of BMDMs from WT and GNMT^−/− mice incubated with or without oxLDL (50 αg/mL) for 24 h. Magnification: 40×. Levels of cholesterol and triglycerides analyzed by colorimetric assay kits. B) BMDMs from WT and GNMT^−/− mice were treated with vehicle or TNF-α (10 ng/mL) for 24 h. After incubation, cell culture media was collected and the production of IL-6, MCP-1 and MIP-2 was evaluated by ELISA. Data are mean ± SEM from 5 independent experiments. *P < 0.05 versus WT vehicle-treated group, #P < 0.05 versus WT oxLDL-treated or TNF-α-treated group.