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Figure 1 | Molecular Medicine

Figure 1

From: G Protein-Coupled Receptor Kinase 6 Acts as a Critical Regulator of Cytokine-Induced Hyperalgesia by Promoting Phosphatidylinositol 3-Kinase and Inhibiting p38 Signaling

Figure 1

Increased and prolonged IL-1β-induced thermal hyperalgesia and mechanical allodynia in GRK6−/− mice. (A) Heat withdrawal latencies were determined using the Hargreaves test at three different intensities (n = 8). (B) Thresholds to mechanical stimulation were determined using von Frey hairs (n = 14). (C) WT and GRK6−/− mice (n = 8–12) received an intraplantar injection of IL-1β, and the decrease in heat withdrawal latency was determined 2 h after injection. Two-way ANOVA: genotype: P < 0.001; dose: P < 0.001; interaction: P < 0.001. (D, E) Time course of IL-1β-induced thermal hyperalgesia in WT and GRK6−/− mice (D: 10 pg IL-1β; E: 1,000 pg IL-1β; n = 8–14). (F) Time course of IL-1β-induced mechanical allodynia in WT and GRK6−/− mice (n = 8). Data are expressed as mean ± SEM. *P < 0.05; **P < 0.01, ***P < 0.001.

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