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Figure 5 | Molecular Medicine

Figure 5

From: G Protein-Coupled Receptor Kinase 6 Acts as a Critical Regulator of Cytokine-Induced Hyperalgesia by Promoting Phosphatidylinositol 3-Kinase and Inhibiting p38 Signaling

Figure 5

Role of p38 and PI 3-kinase in TNF-α-induced thermal hyperalgesia in GRK6−/− mice. (A) Mice received an intraplantar injection of TNF-α (100 pg/paw; n = 8), and the change in heat withdrawal latency was determined over time. Mice were injected intra-plantarly with the p38 inhibitor SB239063 (B) or the PI 3-kinase inhibitor LY249002 (C) 20 min before intraplantar injection of TNF-α (100 pg/paw; n = 4). Heat withdrawal latencies were determined over time. (D) WT and GRK6−/− mice (n = 8) received an intraplantar injection of PGE2 (100 ng/2.5 µL), and the decrease in heat withdrawal latency was determined over time. All data are expressed as mean ± SEM. GRK6−/−, vehicle, versus GRK6−/−, treatment: *P < 0.05, **P < 0.01, ***P < 0.001. WT, vehicle, versus WT, treatment: #P < 0.05, ##P < 0.01, ###P < 0.001.

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