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Figure 1 | Molecular Medicine

Figure 1

From: Toll-Like Receptor 4 Engagement Inhibits Adenosine 5′-Monophosphate-Activated Protein Kinase Activation through a High Mobility Group Box 1 Protein-Dependent Mechanism

Figure 1

TLR4 engagement diminishes AMPK activation. Bone marrow neutrophils (A, B, D and E) or RAW 274.7 macrophages (F, G, H and I) were treated with LPS (0 or 100 ng/mL) for 4 h followed by culture with AICAR (0, 100, 250, 500 or 1,000 µmol/L) for an additional 90 min or H2O2 (0, 100, 150, 200 or 250 ¿mol/L) for 45 min, as indicated. Representative Western blots and mean optical densitometry show amount of AMPK, phospho-Thr172AMPKα, ACC, phospho-Ser79ACC and actin. Data are means ± standard deviation (SD). Two independent experiments were performed. (C) Average of AMP-to-ATP ratio in neutrophils cultured with or without LPS (100 ng/mL) for 4 h (means ± SD, n = 4 independent experiments, *P < 0.05).

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Molecular Medicine

ISSN: 1528-3658