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Figure 3 | Molecular Medicine

Figure 3

From: Toll-Like Receptor 4 Engagement Inhibits Adenosine 5′-Monophosphate-Activated Protein Kinase Activation through a High Mobility Group Box 1 Protein-Dependent Mechanism

Figure 3

Effects of HMGB1 expression on AMPK activity. (A-D) The amounts of phosphorylated AMPK, ACC, total AMPK, ACC, HMGB1 and HMGB1-FLAG were determined in MCF7-HMGB1 cells incubated without (−DOX) or with doxycycline (+DOX) for 24 h followed by exposure to AICAR (A, B) or H2O2 (C, D) for 1 h. Representative Western blots are shown. The amount of phospho-AMPK and phospho-ACC (B, D) were obtained from three independent experiments (means ± SD, *P < 0.05, **P < 0.01, ***P < 0.001).

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